We have expanded the previous NCCC Irradiation Shared Resource (137Cs irradiator and 6-18 MeV linear accelerator) to include Optical Cellular Microscopy (Confocal Microscopy) and Electron Microscopy, as well as state-of-the-art Preclinical Imaging (large and small animal). The new resource now is called the Irradiation, Preclinical Imaging, & Microscopy (IPIM) Shared Resource. The IPIM mission is to use these facilities to support investigators in solving basic and clinical cancer research questions. It is also our goal to promote NCCC investigator awareness of these facilities and to continue to provide the most sophisticated and relevant technologies to NCCC and associated investigators. All resource staff are committed individually to the generation of the highest quality and most meaningful results through personalized instruction and support of each project.
2 area survey monitors.
FEI Company Tecnai F20ST 200kV field emission gun transmission electron microscope with EDAX X-ray microanalysis system, and two AMT digital cameras – a side mount 1kx1k pixel camera and a bottom mount 2kx2k pixel camera. Point resolution 0.24nm, lattice resolution 0.1nm.
FEI Company XL-30 environmental field emission gun scanning electron microscope with EDAX X-ray microanalysis system. Imaging with secondary or backscattered electrons.
Primary use: highly detailed in-vivo imaging studies.
Primary use: highly detailed ex-vivo imaging studies.
Used for large and small-animal imaging studies.
This workstation has a high-end graphics card and 27-inch monitor to augment the older Dell 650 Xeon workstation. Volocity, AutoDeblur/AutoQuant, Image-J, and FIji software are available for image processing and analysis.
JEOL JEM-1010 100kV transmission electron microscope with LaB6 emitter, high contrast objective lens. Equipped with bottom mount 2kx2k pixel AMT digital camera. Point resolution 0.45nm, lattice resolution 0.2nm.
This new fluorescence microscope was acquired to meet requests from NCCC and Geisel School of Medicine (GSM) investigators. The microscope has a wide assortment of fluorescence filters and objectives making it very versatile. Visualization and high quality image capture of slides/multi-well plates using immunohistochemistry (IHC) or fluorescence readily is achieved.
The inverted microscope allows simultaneous 4-color imaging (404, 491, 561, 640nm lasers). The system is equipped with stage top live cell incubation, zero drift focus control, an Andor 2- camera-imaging adapter, and two Andor Zyla 5.5 cameras and is controlled using Metamorph software.
Primary use: pre-clinical rodent and large-animal imaging studies and MRI.
Primary use: small-animal radioisotope based molecular imaging.
"Exradin Farmer-type Chambers are specifically designed for absolute dosimetry calibrations in water, air, or other phantom material. The Model A12 is completely characterized in TG 51 and TRS 398."
Primary use: pre-clinical rodent imaging studies.
2 hand-held electrometers.
Primary use: high detail real-time ultrasound studies, cardiac output and tumor volume estimates.
Primary use: imaging bioluminescent and fluorescent reporters both in-vivo and in-vitro.
This microscope allows low magnification stereoscopic viewing (0.6X-1.6X objectives) or monocular viewing (10X-20X) with an 11X zoom. Image capture is achieved with a Zeiss MR-AxioCam CCD camera or a PixelView color CCD camera.
The system has four lasers (Diode 405nm, Argon Gas 458-477- 488-514nm, Hene 543nm, and Hene 633nm), two side-on PMT detectors, and a “META” light sensor, capable of spectral imaging on a 32-element PMT detector. The instrument performs confocal imaging of live cells, fixed cells, and tissues. The system has software for 3-D reconstruction, co-localization, and kinetic analysis of
cell function from acquired images. Live cell imaging is achieved using a temperature-controlled stage insert and an objective heater.