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Shared Instruments Core Laboratory





  • ABI 7500 Fast real-time PCR system ( Real-time PCR machine )

    SU Group 223483 & 223484. Lab has two of these instruments. "The Applied Biosystems 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes.

    * Specially designed Fast block ensures thermal uniformity at top speeds
    * Faster ramp rates enable rapid results without compromising extension times or assay quality
    * Fast optical plates ensure excellent precision in 10-30 uL reaction volumes
    * Use TaqMan Fast Universal PCR Master Mix to run multiple Fast assays on one plate
    * Finish more runs per day with the excellent performance equivalent to that offered by the 7500 System"

  • ABI MALDI MS ( Mass spectrometer )

  • Agilent 2100 Bioanalyzer ( Microfluidics platform )

    "The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells on a single platform. Results are delivered within 30-40 minutes in automated, high quality digital data."

  • ChemiDoc XRS+ ( Imager )

  • Kodak X-Omat 2000A ( X-ray film processor )

  • NanoDrop spectrophotometer ( Spectrophotometer )

  • StepOne+ Realtime Q-PCR ( Thermal cycler )

    96 well format

  • Storm 860 imager ( Imager )

    SU Group 205215.

  • Typhoon 8600 imager ( Imager )

  • Typhoon 9410 imager ( Imager )



  • 2100 Expert software ( Software )

    "The 2100 Expert Software for the Agilent 2100 Bioanalyzer provides easy instrument set-up and run control, and offers powerful data evaluation tools for all available assays, automatic and manual integration, smear analysis and many assay-specific tools."

  • 7500 Fast ( Software )

    Visualization of qPCR data

  • ABI Primer Express ( Software )

    "The Primer Express® Software v3.0 allows you to design your own primers and probes using TaqMan® and SYBR® Green I dye chemistries for gene quantitation and allelic discrimination (SNP) real-time PCR applications. Developed specifically for use with our 7300, 7500, 7500 Fast, and 7900HT Fast Real-Time PCR Systems, Primer Express® Software provides customized application-specific documents for absolute/relative quantitation and allelic discrimination."

  • Data Explorer ( Software )

    Mass spectrometer instrument control and data analysis software.

  • ImageQuant TL ( Software )

    "ImageQuant™ TL is the most automatic and easy-to-use general image analysis software. It offers four separate functional areas within one product:

    * 1-D electrophoresis gels.
    * Dot blots, microplates, and basic arrays.
    * Colony counting and measurement of 2-D spots and other image features.
    * Toolbox to analyze gels with user-defined objects.

    Its high level of automation throughout allows for fully automatic analysis of 1-D gels - including lane creation, background subtraction, band detection, molecular weight calibration, quantity calibration, and normalization - in a matter of a few seconds. This ensures high reproducibility. "

  • NanoDrop ( Software )

    "Classic NanoDrop Applications:
    * Nucleic Acid
    * Protein A280
    * Micro Array
    * Proteins & Labels
    * BCA, Bradford, Lowry, Pierce 660 nm
    * UV-Vis
    * Cell Cultures
    * plus Diagnostics"

  • Quantity One ( Software )

    "Image acquisition and analysis software for use with Bio-Rad imaging systems"

  • Voyager ( Software )

    Mass spectrometer instrument control and data analysis software.

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Last updated: 2012-10-11T17:01:39.796-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016