The Spaller lab specializes in the study of protein-ligand and protein-protein interactions of biological and medicinal significance, focusing on those that regulate oncogenic and neuronal signal transduction pathways. Our efforts encompass aspects that are both fundamental—investigating the underlying molecular and cellular mechanisms—and applied—developing reagents that will serve as molecular probes in biological studies, or as compounds for use in drug development.
Biacore is known for protein interaction analysis , including small molecule drug candidates; used in areas such as pharmaceutical drug discovery, antibody characterization, proteomics, immunogenicity, biotherapeutic development and manufacture...
Protein interaction research
• Define structure/function relationships
• Understand the dynamics of molecular pathways
• Find and validate potential drug targets or diagnostic markers
• Develop and run assays for interactions involving LMW compounds
• Select better research tools, diagnostics and therapeutics
Label-free protein interaction analysis provides unique and comprehensive data on the interactions between biomolecules
• Quantitative kinetic analysis (rate constants)
• Quantitative determination of affinity constants
• Determination of binding specificity (yes/no)
• Concentration analysis
Design, synthesis, purification and characterization of peptides. These peptides can be prepared synthetically as isolated compounds, or in a biological platform (i.e., phage display). Such peptides can be designed for use as in vitro or in vivo probes to target proteins of interest. Depending on the nature of the protein target, and its cellular function and activity, these peptides might serve as either inhibitors, activators, agonists, or antagonists. The peptides can be chemically modified to alter properties of interest; these include binding affinity, selectivity, cellular stability, cellular permeability, fluorescence, and biotinylation. In addition to serving as biologically active agents, peptide compounds could be used to aid the development of biochemical assays, such as for high-throughput screening applications.