eagle-i Dartmouth CollegeDartmouth College
See it in Search

Shworak Laboratory

Affiliations:

People:

Resources:

Instruments

Organisms and Viruses

  • 129S4/SvJae ( Mus musculus )

    Not commercially available; inbred.

  • ATm/m ( Mus musculus )

    Knock-in.

  • Hs3st1-/- ( Mus musculus )

    Normal levels of anticoagulant heparan sulfate are not essential for normal hemostasis.

    Complete excision of Hs3st1 coding region.

  • sh-Luciferase ( Lentivirus )

  • sh-THADA433 ( Lentivirus )

    Expresses shRNA against THADA.

  • sh-THADA434 ( Lentivirus )

    Expresses shRNA against THADA.

  • sh-THADA435 ( Lentivirus )

    Expresses shRNA against THADA.

  • sh-THADA436 ( Lentivirus )

    Expresses shRNA against THADA.

  • sh-THADA437 ( Lentivirus )

    Expresses shRNA against THADA.

Reagents

  • 3OST1 ( Expression construct )

  • CHO ( Tumor-derived cell line )

  • CHO-3OST1 ( Tumor-derived cell line )

    Selectable marker: Neomycin.

  • CHO-hTHADA ( Tumor-derived cell line )

    Selectable marker: Neomycin

    Overexpresses hTHADA.

  • EA shTH3 ( Tumor-derived cell line )

    Selectable marker: Puromycin. Hybrid line of HUVEC and A549. EA.hy926 stably expressing THADA shRNA3.

  • EA shLuc1 ( Tumor-derived cell line )

    Selectable marker: Puromycin. Hybrid line of HUVEC and A549. EA.hy926 stable expressing luciferase shRNA c1.

  • EA shLuc2 ( Tumor-derived cell line )

    EA.hy926 stable expressing Luciferase shRNA c2. Selectable marker: Puromycin. Hybrid line of HUVEC and A549.

  • EA shTH4 ( Tumor-derived cell line )

    Selectable marker: Puromycin. Hybrid line of HUVEC and A549. EA.hy926 stable expressing THADA shRNA4.

  • EA shTH5 ( Tumor-derived cell line )

    Selectable marker: Puromycin. EA.hy926 stably expressing THADA shRNA5. Hybrid line of HUVEC and A549.

  • EA shTH6 ( Tumor-derived cell line )

    Selectable marker: Puromycin. Hybrid line of HUVEC and A549. EA.hy926 stable expressing THADA shRNA6.

  • EA shTH7 ( Tumor-derived cell line )

    Selectable marker: Puromycin. Hybrid line of HUVEC and A549. EA.hy926 stable expressing THADA shRNA7.

  • EA.hy926 ( Tumor-derived cell line )

    Hybrid line of HUVEC and A549.

  • HAEC ( Cell line )

  • HEK 293T ( Tumor-derived cell line )

  • Library set 1, Library 1 ( Known bioactives collection chemical library )

    cDNA from size fractionated mRNA (isolated from COS20 cells) cloned into Not-I site of pcDNA-1 expression vector; bugstrain unknown. Input mRNA size fraction: > 0.3 kb
    Mean insert size: 0.4
    Fold overrepresentation: 106

  • Library set 1, Library 2 ( Known bioactives collection chemical library )

    cDNA from size fractionated mRNA (isolated from COS20 cells) cloned into Not-I site of pcDNA-1 expression vector; bugstrain unknown. mRNA size fraction: >1.1 kb
    Mean insert size: 1.2 kb
    Fold overrepresentation: 22

  • Library set 1, Library 3 ( Known bioactives collection chemical library )

    cDNA from size fractionated mRNA (isolated from COS20 cells) cloned into Not-I site of pcDNA-1 expression vector; bugstrain unknown. Input mRNA size fraction: > 4.0 kb
    Mean insert size: 0.4 kb
    Fold overrepresentation: 18

  • Library set 1, Library 4 ( Known bioactives collection chemical library )

    cDNA from size fractionated mRNA (isolated from COS20 cells) cloned into Not-I site of pcDNA-1 expression vector; bugstrain unknown. Input mRNA size fraction: 2.6-4 kb
    Mean insert size: 0.4 kb
    Fold overrepresentation: 5

  • Library set 1, Library 5 ( Known bioactives collection chemical library )

    cDNA from size fractionated mRNA (isolated from COS20 cells) cloned into Not-I site of pcDNA-1 expression vector; bugstrain unknown. Input mRNA size fraction: 1.5-2.6 kb
    Mean insert size: 0.4 kb
    Fold overrepresentation: 4

  • Library set 2, HUVEC cell line ( Known bioactives collection chemical library )

    Fold overrepresentation 5-10.

    Library amplified in Escherichia coli XL1-Blue MRF9.

    Description of cell line: Primary human umbilical vein endothelial cells.

    Concentration of amplified library: 2.30E+09 pfu/ul polyA+ RNA from different cell types were cloned into lambda-Zap Express cDNA library (Stratagene).

  • Library set 2, LA-9 cell line ( Known bioactives collection chemical library )

    Fold overrepresentation 5-10

    Library amplified in Escherichia coli XL1-Blue MRF9.

    Description of cell line: L-cell line with undetectable HSAT+ expression.

    Concentration of amplified library: 1.30E+09 pfu/ul polyA+ RNA from different cell types were cloned into lambda-Zap Express cDNA library (Stratagene).

  • Library set 2, LTA cell line ( Known bioactives collection chemical library )

    Fold overrepresentation 5-10

    Library amplified in Escherichia coli XL1-Blue MRF9.

    Description of cell line: L-cell clone with high HSAT+ expression.

    Concentration of amplified library: 1.70E+09 pfu/ul polyA+ RNA from different cell types were cloned into lambda-Zap Express cDNA library (Stratagene).

  • Library set 2, LTA F4 cell line ( Known bioactives collection chemical library )

    Fold overrepresentation 5-10

    Library amplified in Escherichia coli XL1-Blue MRF9.

    Description of cell line: 2.3-4 kb size fractionated mRNA from LTA cells.

    Concentration of amplified library: 1.30E+06 pfu/ul polyA+ RNA from different cell types were cloned into lambda-Zap Express cDNA library (Stratagene).

  • Library set 2, VI-7 cell line ( Known bioactives collection chemical library )

    Fold overrepresentation 5-10

    library amplified in Escherichia coli XL1-Blue MRF9.

    Description of cell line: Clone from chemical mutagenesis of LTA, low HSAT+ expression.

    Concentration of amplified library: 1.00E+10 pfu/ul polyA+ RNA from different cell types were cloned into lambda-Zap Express cDNA library (Stratagene).

  • pDEST12.2-hTHADA ( Plasmid )

    Thyroid adenoma associated.

  • pLKO.1-puro ( Lentiviral plasmid )

    Luciferase expressing vector.

  • pLKO.1-puro ( Lentiviral plasmid )

  • RFPEC ( Cell line )

  • THADA ( Expression construct )


Last updated: 2012-12-04T14:12:57.113-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016