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Barlowe Laboratory


The research program is focused on understanding protein and lipid transport through the early secretory pathway in eukaryotic cells. This is an essential process that initiates the delivery of proteins to intracellular organelles and to the cell surface for secretion. Because of the advantage for genetic analyses, we investigate this process mainly in the yeast Saccharomyces cerevisiae. Our primary interest is elucidating the molecular mechanisms that underlie vesicular transport between the endoplasmic reticulum (ER) and Golgi complex. Transport between these compartments is mediated by membrane vesicles, termed COPII vesicles, that bud from the ER and fuse with and/or form the Golgi complex. Our studies combine molecular genetics, proteomics and microscopy with cell free assays that measure COPII-dependent transport. This cell free transport reaction proceeds through the biochemically distinct stages of COPII-dependent vesicle budding, Uso1p-dependent vesicle tethering and SNARE protein-dependent membrane fusion. We have reproduced these stages with isolated membranes and purified soluble molecules. A long-term goal of my research program is to reconstitute distinct sub-reactions in ER/Golgi transport with defined protein and lipid fractions for elucidation of catalytic mechanisms.




Organisms and Viruses

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Last updated: 2013-08-09T11:19:41.632-04:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016