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Sanchez Laboratory

Summary:

"Cells have evolved intricate networks of proteins or signal transduction pathways that allow them to integrate internal or external signals in order to respond to specific cues during development and during stress. Molecules that originate from neighboring cells, tissues, and pathogens, can act over short and long distances to elicit a physiological response. Signaling pathways can also be triggered within the cell, as is the case when cells are placed under alert following DNA damage or when there is a problem with DNA replication. Such information is transmitted via protein-protein interactions and protein modifications and leads to the alteration of gene expression and other events that regulate cell division, DNA repair or cell death. Our laboratory focuses on checkpoint signaling events triggered by DNA damage or replication interference. We are taking genetic, biochemical and cell biological approaches to study these signaling pathways. We are interested in dissecting signaling complexes involved in the response to lesions that are caused by DNA damaging agents or stalled replication forks and determining how specific protein-protein interactions and cellular location are regulated to transmit this information. We are also interested in the interactions between the signaling molecules and the enzymes that function in DNA replication and repair.

The pathways that we study are involved in both the etiology and treatment of cancer. Loss-of-function mutations in mammalian checkpoint genes compromise the response to DNA damage at the cellular level and at the level of the organism lead to a predisposition to cancer. In addition, cancer therapies frequently rely on drugs or agents that trigger genomic instability by taking advantage of the fact that cancer cells have defects in the response to DNA damage."

For a description of projects, see research: http://www.dartmouth.edu/~sanchezlab/

Affiliations:

People:

Resources:

Organisms and Viruses

  • 3468* ( Saccharomyces cerevisiae )

    Genotype:
    As BY4741 mrc1 KanMX

  • 3578* ( Saccharomyces cerevisiae )

    Genotype:
    As BY4741 rad9 KanMX

  • 512* ( Saccharomyces cerevisiae )

    Genotype:
    As BY4741 sml1 KanMX

  • BY4741 ( Saccharomyces cerevisiae )

    Genotype:
    MATa his3 1 leu2 0 met15 0 ura3 0

  • Dmec1 + GAP:DUN1 ( Saccharomyces cerevisiae )

    NO: C8.

    Genotype: haploid from YWJ60 (MEC+/Dmec1::HIS3) + PZZ74 (GAP:DUN1:URA3).

  • JBY257 ( Saccharomyces cerevisiae )

    NO: B5. Genotype: MATa cdc13-1.

  • JBY397 ( Saccharomyces cerevisiae )

    NO: A3. Genotype: MATa PDS1-3XHA-URA3.

  • JBY400 ( Saccharomyces cerevisiae )

    NO: A4. Genotype: MATa cdc13-1 PDS1-3XHA-URA3.

  • JBY417-4d ( Saccharomyces cerevisiae )

    Genotype: MATa cdc13-1 rad53-21 PDS1-3XHA-URA3. NO: A6.

  • JBY418-11d ( Saccharomyces cerevisiae )

    NO: A5. Genotype: MATa cdc13-1 chk1∆::HIS3 PDS1-3XHA-URA3.

  • JBY428 ( Saccharomyces cerevisiae )

    Genotype: MATa trp1-1Δ::lacO-TRP1::lacO-LEU2 his3-11,15::GFP-LacI-HIS3. NO: B6.

  • JBY432 ( Saccharomyces cerevisiae )

    Genotype: MATa cdc13-1 trp1-1::lacO-TRP1::lacO-LEU2 his3-11,15::GFP-LacI-HIS3. NO: B7.

  • JBY446 ( Saccharomyces cerevisiae )

    NO: B8. Genotype: MATa cdc13-1 rad53-21 trp1-1::lacO-TRP1::lacO-LEU2 his3-11,15::GFP-LacI-HIS3.

  • JBY457 ( Saccharomyces cerevisiae )

    NO: B9. Genotype: MATa cdc13-1 mec1∆::HIS3 trp1-1::GAP-RNR1-TRP1 PDS1-3XHA-URA3.

  • JBY460 ( Saccharomyces cerevisiae )

    Genotype: MATa cdc13-1 rad53∆::HIS3 trp1-1::GAP-RNR1-TRP1 PDS1-3XHA-URA3. NO: C1.

  • JBY463 ( Saccharomyces cerevisiae )

    Genotype: MATa cdc13-1 pds1∆::LEU2. NO: C2.

  • JBY516 ( Saccharomyces cerevisiae )

    NO: C3. Genotype: MATa cdc13-1 chk1∆::HIS3.

  • JBY520 ( Saccharomyces cerevisiae )

    NO: C6. Genotype: cdc13-1Dchk1rad53-21 LEU+ in Y300 MATa.

  • JBY522-25b ( Saccharomyces cerevisiae )

    NO: A7. Genotype: MATa cdc13-1 chk1∆::HIS3 rad53-21 PDS1-3XHA-URA3.

  • JBY523 ( Saccharomyces cerevisiae )

    Genotype: MATa cdc13-1 chk1∆::HIS3 trp1-1::lacO-TRP1::lacO-LEU2 his3-11,15::GFP-LacI-HIS3. NO: C4.

  • MDW552 ( Saccharomyces cerevisiae )

    Genotype:
    ira2∆::URA3 nab3-11

  • MDW561 ( Saccharomyces cerevisiae )

    Genotype:
    erg6∆::URA3 nab3∆::LEU2 nab3-3

  • MDW578 ( Saccharomyces cerevisiae )

    Genotype:
    ctk1∆::KANMX erg6∆::URA3

  • MLY41a ( Saccharomyces cerevisiae )

    Genotype:
    ura3-52

  • Y300 ( Saccharomyces cerevisiae )

    Genotype:
    MATa ade2-1 trp1-1 ura3-1 leu2-3,112 his 3-11,15 can1-100

  • Y301 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 rad53-21

  • Y578 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 dun1Δ::HIS3

  • Y578 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 dun1 HIS3

  • Y581 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 mec1Δ::HIS3 trp1-1::GAP-RNR1-TRP1

  • Y581 ( Saccharomyces cerevisiae )

    NO: B9. Genotype: Brian Genes and Dev. 1998 Dmec1:HIS3 TRP GAP-RNR1.

  • Y604 ( Saccharomyces cerevisiae )

    Genotype: Brian mec1-21 MATa.

    NO: C2.

  • Y606 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 rad53Δ::HIS3 trp1-1::GAP-RNR1-TRP1

  • Y654 ( Saccharomyces cerevisiae )

    Genotype: Brian Dtel1::HIS3 MATa.

    NO: C1.

  • Y657 ( Saccharomyces cerevisiae )

    Genotype: WT MATa Y300 background.

    NO: A1.

  • Y658 ( Saccharomyces cerevisiae )

    NO: A2.

    Genotype: Dtel1:HIS3 MATa Y300 background.

  • Y659 ( Saccharomyces cerevisiae )

    NO: A3.

    Genotype: rad53-21 MATa Y300 background.

  • Y660 ( Saccharomyces cerevisiae )

    NO: A4.

    Genotype: rad53-21Dtel1:HIS3 MATa Y300 background.

  • Y663 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 mec1-21

  • Y663 ( Saccharomyces cerevisiae )

    Genotype: mec1-21 MATa Y300 background.

    NO: A5.

  • Y664 ( Saccharomyces cerevisiae )

    Genotype: mec1-21Dtel1:HIS3 MATa Y300 background.

    NO: A6.

  • Y677 ( Saccharomyces cerevisiae )

    NO: C5. Genotype: Drad53:HIS3 + GAL-RNR1 2un TRP in Y300 .

  • Y801 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1Δ::HIS3

  • Y801 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1 HIS3

  • Y808 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 PDS1 3XHA-PDS1-URA3

  • Y811 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 cdc13-1 chk1Δ::HIS3 PDS1-3XHA::URA3

  • Y816 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 cdc13-1

  • Y818 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 cdc13-1 chk1Δ::HIS3

  • Y857 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1 URA3 dun1 HIS3

  • YHC200 ( Saccharomyces cerevisiae )

    Genotype:
    As Y816 chk1Δ::URA3

  • YHC202 ( Saccharomyces cerevisiae )

    Genotype:
    As Y816 chk1-ΔGD::URA3

  • YHC205 ( Saccharomyces cerevisiae )

    Genotype:
    As Y816 chk1-G503D::URA3

  • YHC206 ( Saccharomyces cerevisiae )

    Genotype:
    As Y816 chk1-L506R::URA3

  • YHC208 ( Saccharomyces cerevisiae )

    Genotype:
    As Y816 chk1-ΔTKF::URA3

  • YHC212 ( Saccharomyces cerevisiae )

    Genotype:
    As Y816 chk1-F419A::URA3

  • YHC232 ( Saccharomyces cerevisiae )

    Genotype:
    As Y816 chk1-3AQ::URA3

  • YHC234 ( Saccharomyces cerevisiae )

    Genotype:
    As Y816 chk1-L506R, 3AQ::URA3

  • YHC300 ( Saccharomyces cerevisiae )

    Genotype:
    As Y578 chk1 ::URA3

  • YHC302r ( Saccharomyces cerevisiae )

    Genotype:
    As Y578 chk1-ΔGD::URA3

  • YHC305 ( Saccharomyces cerevisiae )

    Genotype:
    As Y578 chk1-G503D::URA3

  • YHC308 ( Saccharomyces cerevisiae )

    Genotype:
    As Y578 chk1-ΔTKF::URA3

  • YHC312 ( Saccharomyces cerevisiae )

    Genotype:
    As Y578 chk1-F419A::URA3

  • YHC332 ( Saccharomyces cerevisiae )

    Genotype:
    As Y578 chk1-3AQ::URA3

  • YHC334 ( Saccharomyces cerevisiae )

    Genotype:
    As Y578 chk1-L506R, 3AQ::URA3

  • YHC335 ( Saccharomyces cerevisiae )

    Genotype:
    As Y578 chk1-T421A::URA3

  • YHC336 ( Saccharomyces cerevisiae )

    Genotype:
    As Y578 chk1-L506R, T421A::URA3

  • YJP1 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1 HIS3 PDS1 3XHA-PDS1-URA3

  • YJP1124 ( Saccharomyces cerevisiae )

    Genotype:
    As Y816 chk1-L506R, 3AQ::URA3

  • YJP1129 ( Saccharomyces cerevisiae )

    Genotype:
    As Y578 chk1-L506R::URA3 rad9Δ::HIS3

  • YJP1130 ( Saccharomyces cerevisiae )

    Genotype:
    As Y578 chk1-L506R, 3AQ::URA3 rad9Δ::HIS3

  • YJP1150 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1Δ::HIS3 dun1Δ::HIS3 tof1Δ

  • YJP1159 ( Saccharomyces cerevisiae )

    Genotype:
    As Y816 chk1-L506R::URA3 rad9Δ::HIS3

  • YJP1160 ( Saccharomyces cerevisiae )

    Genotype:
    As Y816 chk1-L506R, 3AQ::URA3 rad9Δ::HIS3

  • YJP2 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 dun1 HIS3 PDS1 3XHA-PDS1-URA3

  • YJP219 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1 ::URA3 rad9 ::HIS3

  • YJP275 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 rpd3Δ::URA3

  • YJP278 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1Δ::HIS3 rpd3Δ::URA3

  • YJP281 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 dun1Δ::HIS3 rpd3Δ::URA3

  • YJP285 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1Δ::HIS3 dun1Δ::HIS3 rpd3Δ::URA3

  • YJP3 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 sml1 KanMX

  • YJP321 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 dun1Δ::HIS3 rad9Δ::HIS3

  • YJP328 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 CHK1::CHK1-HA-URA3

  • YJP328 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 CHK1 HA-CHK1-URA3

  • YJP341 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 dun1Δ::HIS3 CHK1::CHK1-HA-URA3

    Generated by crossing Y578 with YJP328.

  • YJP341 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 dun1 HIS3 CHK1 HA-CHK1-URA3

  • YJP343 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 mrc1Δ::kanMX CHK1::CHK1-HA-URA3

  • YJP349 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 mrc1Δ::kanMX CHK1::CHK1-HA-URA3

  • YJP356 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 rad9Δ::HIS3 CHK1::CHK1-HA-URA3

  • YJP4 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1 HIS3 sml1 KanMX

  • YJP457 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1Δ::URA3 dun1Δ::HIS3 rad9Δ::HIS3

  • YJP5 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 dun1 HIS3 sml1 KanMX

  • YJP528 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 dun1Δ::HIS3 mre11Δ::kanMX CHK1::CHK1-HA-URA3

  • YJP534 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 mre11Δ::kanMX CHK1::CHK1-HA-URA3

  • YJP554 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1Δ::URA3 dun1Δ::HIS3 sml1Δ::URA3

  • YJP6 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1 HIS3 dun1 HIS3 sml1 KanMX

  • YJP667 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1Δ::URA3 dun1Δ::HIS3 mrc1Δ::kanMX sml1Δ::URA3

  • YJP7 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1 HIS3 dun1 HIS3 PDS1 3XHA-PDS1-URA3

  • YJP8 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1Δ::HIS3 dun1Δ::HIS3

  • YJP8 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1 HIS3 dun1 HIS3

  • YJP875 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 dun1Δ::HIS3 tof1Δ::URA3

  • YJP9 ( Saccharomyces cerevisiae )

    Genotype:
    As Y81 chk1 HIS3 dun1 HIS3

  • YJP947 ( Saccharomyces cerevisiae )

    Genotype:
    chk1Δ::HIS3 dun1Δ::HIS3 tof1Δ::URA3

  • YJP979 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 clb5Δ::kanMX

  • YJP980 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1Δ::URA3 clb5Δ::kanMX

  • YJP981 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 dun1Δ::URA3 clb5Δ::kanMX

  • YJP982 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1Δ::HIS3 dun1Δ::HIS3 clb5Δ::kanMX

  • YKLS12 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1 URA3 rad52 URA3

  • YKLS13 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 dun1 HIS3 rad52 URA3

  • YKLS14 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 chk1 URA3 dun1 HIS3 rad52 URA3

  • YKLS16 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 cdc15-2 dun1 HIS3

  • YKLS17 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 cdc15-2 chk1 URA3 dun1 HIS3

  • YKLS18 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 cdc15-2 rad53-21

  • YKLS26 ( Saccharomyces cerevisiae )

    Generated by crossing Y857 with KRY70 (rad52 LEU2), generously provided by Thomas Petes.

    Genotype:
    As Y300 rad52 URA3

  • YKLS27 ( Saccharomyces cerevisiae )

    Generated by crossing YJP9 with Y808.

    Genotype:
    As Y300 cdc15-2

  • YKLS27 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 cdc15-2

    Generated by crossing Y81 with K1993 (cdc15-2), generously provided by Stephen Elledge.

  • YKLS29 ( Saccharomyces cerevisiae )

    Generated by crossing 3468 with YJP341.

    Genotype:
    As Y300 dun1 HIS3 mrc1 KanMX CHK1 HA-CHK1-URA3

  • YMW142 ( Saccharomyces cerevisiae )

    Genotype:
    As Y816 ira2Δ::URA3

  • YMW152 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 ira2Δ::URA3

  • YMW197 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 ira1Δ::URA3

  • YMW198 ( Saccharomyces cerevisiae )

    Genotype:
    As Y816 ira1Δ::URA3

  • YMW199 ( Saccharomyces cerevisiae )

    Genotype:
    As Y816 ira1Δ::URA3 ira2Δ::URA3

  • YMW203 ( Saccharomyces cerevisiae )

    Genotype:
    cdc13-1 chk1Δ::HIS3 ira1Δ::URA3ira2Δ::URA3

  • YMW208 ( Saccharomyces cerevisiae )

    Genotype:
    As Y300 ira1Δ::URA3 ira2Δ::URA3

  • YMW257 ( Saccharomyces cerevisiae )

    Genotype:
    cdc13-1 tpk2Δ::KAN

  • YMW446 ( Saccharomyces cerevisiae )

    Genotype:
    cdc13-1/CDC13 ira1Δ::URA3/IRA1ira2Δ::URA3/IRA2 a/α

  • YMW461 ( Saccharomyces cerevisiae )

    Genotype:
    cdc13-1 ira1Δ::URA3 ira2Δ::URA3 rad53-21

  • YMW512 ( Saccharomyces cerevisiae )

    Genotype:
    cdc13-1 ira1Δ::URA3 ira2Δ::URA3tpk2Δ::KAN

  • YMW534 ( Saccharomyces cerevisiae )

    Genotype:
    cdc13-1 [pMW034]

  • YMW535 ( Saccharomyces cerevisiae )

    Genotype:
    cdc13-1 [pMW036]

  • YMW536 ( Saccharomyces cerevisiae )

    Genotype:
    cdc13-1 ira1Δ::URA3 ira2Δ::URA3 [pMW034]

  • YMW537 ( Saccharomyces cerevisiae )

    Genotype:
    cdc13-1 ira1Δ::URA3 ira2Δ::URA3 [pMW036]

  • YMW544 ( Saccharomyces cerevisiae )

    Genotype:
    cdc13-1 pds1Δ::LEU2

  • YMW545 ( Saccharomyces cerevisiae )

    Genotype:
    cdc13-1 ira1Δ::URA3 ira2Δ::URA3pds1Δ::LEU2

  • YS110 ( Saccharomyces cerevisiae )

    NO: A7. Genotype: Ddun1:HIS3 Dchk1:URA3 Y300 MATa.

  • YS146 ( Saccharomyces cerevisiae )

    NO: A8. Genotype: Dchk1:HIS3 Y300 MATa.

  • YS146 ( Saccharomyces cerevisiae )

    Genotype: Dchk1:HIS3 MATa Y300 . NO: E8.

  • YS147 ( Saccharomyces cerevisiae )

    NO: E7. Genotype: Y300 + GAL-HACHK1 (pQL10) MATa.

  • YS147 ( Saccharomyces cerevisiae )

    NO: A9. Genotype: Y300 + GAL-HA:CHK1:URA:CEN (pQL10).

  • YS148 ( Saccharomyces cerevisiae )

    NO: A9. Genotype: rad53-21Dchk1:HIS3 Y300 MATa clone 1.

  • YS149 ( Saccharomyces cerevisiae )

    Genotype: rad53-21Dchk1:HIS3 Y300 MATa clone 2. NO: B1.

  • YS167 ( Saccharomyces cerevisiae )

    NO: B1. Genotype: Dchk1::HIS3 in TW397 disruption of CHK1 in TW background MATa.

  • YS168 ( Saccharomyces cerevisiae )

    NO: B2. Genotype: cdc13-1Drad9:LEU2 Dchk1::HIS3 MATa TW background.

  • YS169 ( Saccharomyces cerevisiae )

    NO: B3. Genotype: cdc13-1Drad9:LEU2 Dchk1::HIS3 MATa TW background.

  • YS170 ( Saccharomyces cerevisiae )

    NO: B4. Genotype: cdc13-1Drad9:LEU2 Dchk1::HIS3 MATa TW background.

  • YS175 ( Saccharomyces cerevisiae )

    NO: B8. Genotype: Dpds1:LEU2 in Y300 MATa disrupted pds1 in CRY with pAY55.

  • YS191 ( Saccharomyces cerevisiae )

    NO: B2. Genotype: Ddun1:HIS3 Dchk1:HIS3 Y300 MATa.

  • YS192 ( Saccharomyces cerevisiae )

    Genotype: same as YS191 different isolate. NO: B3.

  • YS193 ( Saccharomyces cerevisiae )

    NO: B4. Genotype: Drad53::HIS3Dchk1::URA3 MATalpha .

  • YS193 ( Saccharomyces cerevisiae )

    NO: E9. Genotype: WT Cry 1 + HA CLB2:URA3:CEN under its own promoter (pMT812).

  • YS194 ( Saccharomyces cerevisiae )

    NO: B5. Genotype: Dchk1:URA3 MATalpha in Y300 ALPHA.

  • YS194 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1 in Y300 (JBY257) + HA CLB2:URA3:CEN under its own promoter (pMT812). NO: F1.

  • YS195 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1rad53-21 + HA CLB2:URA3:CEN under its own promoter (pMT812). NO: F2.

  • YS196 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1 Dchk1 + HA CLB2:URA3:CEN under its own promoter (pMT812). NO: F3.

  • YS197 ( Saccharomyces cerevisiae )

    NO: B6. Genotype: Drad53::HIS3 MATalpha.

  • YS197 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1rad53-21Dchk1::HIS3 (JBY520) + HA CLB2:URA3:CEN under its own promoter (pMT812). NO: F4.

  • YS198 ( Saccharomyces cerevisiae )

    Genotype: Dchk1:URA3 MATa Y300. NO: B7.

  • YS198 ( Saccharomyces cerevisiae )

    NO: F5. Genotype: cdc13-1esp1 in Y300 ALPHA mat alpha .

  • YS199 ( Saccharomyces cerevisiae )

    Genotype: esp1-1 MATalpha in Y300 ALPHA haploid from K2788 (Amon) xY300 ALPHA cross. NO: F6.

  • YS200 ( Saccharomyces cerevisiae )

    NO: F7. Genotype: esp1-1 MATa in Y300 haploid from K2788(Amon) xY300 ALPHA cross.

  • YS201 ( Saccharomyces cerevisiae )

    NO: F8. Genotype: Dcdh1::HIS3 MATa in Y300 haploid from 1120(Amon) xY300 ALPHA cross [1120= Dcdh1::HIS3 PDS1:HADpds1::LEU2 Mata].

  • YS202 ( Saccharomyces cerevisiae )

    NO: F9. Genotype: Dcdh1::HIS3 MATalpha Dpds1::LEU2.

  • YS203 ( Saccharomyces cerevisiae )

    NO: G1. Genotype: cdc13-1Dchk1 MATa in Y300 haploid from YS70xY300.

  • YS204 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1esp-1 mat a in Y300 a2 haploid obtained by mating K2788xcdc13-1 in Y300 ALPHA. NO: G3.

  • YS205 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1 esp1-1 mat a in Y300 a3 haploid obtained by mating K2788xcdc13-1 in Y300 ALPHA. NO: G4.

  • YS206 ( Saccharomyces cerevisiae )

    NO: G5. Genotype: cdc13-1rad53-21 HIS3+ mat a in Y300.

  • YS207 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1Ddun1::HIS3 MATa (YS145) + pRS416 . NO: G6.

  • YS208 ( Saccharomyces cerevisiae )

    NO: G7. Genotype: cdc13-1Ddun1::HIS3 MATa (YS145) + pOC56 GAL-PDS1:URA3.

  • YS209 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1Ddun1::HIS3 MATa (YS145) + pOC57 GAL-dbmpds1:URA3. NO: G8.

  • YS210 ( Saccharomyces cerevisiae )

    NO: G9. Genotype: cdc13-1Ddun1::HIS3 MATa (YS145) + pOC58 GAL-Ddbmpds1:URA3.

  • YS211 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1 Y300 MATa + pRS416 . NO: H1.

  • YS212 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1 Y300 MATa + pOC56. NO: H2.

  • YS213 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1 Y300 MATa + pOC57. NO: H3.

  • YS214 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1 Y300 MATa + pOC58. NO: H4.

  • YS215 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1rad53-21Dcdh1::HIS3 in Y300 MATa (YS238) + pRS416 . NO: H5.

  • YS216 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1rad53-21Dcdh1::HIS3 in Y300 MATa (YS238) + pOC56. NO: H6.

  • YS217 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1rad53-21Dcdh1::HIS3 in Y300 MATa (YS238) + pOC57. NO: H7.

  • YS218 ( Saccharomyces cerevisiae )

    NO: H8. Genotype: cdc13-1rad53-21Dcdh1::HIS3 in Y300 MATa (YS238) + pOC58.

  • YS219 ( Saccharomyces cerevisiae )

    NO: H9. Genotype: cdc13-1Dchk1 (YS203) + pRS416 MAT a in CRY.

  • YS220 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1Dchk1 + pOC56. NO: I1.

  • YS221 ( Saccharomyces cerevisiae )

    NO: I2. Genotype: cdc13-1Dchk1 + pOC57.

  • YS222 ( Saccharomyces cerevisiae )

    NO: I3. Genotype: cdc13-1Dchk1 + pOC58.

  • YS223 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1rad53-21 LEU+ in Y300 MATa + pRS416. NO: I4.

  • YS224 ( Saccharomyces cerevisiae )

    NO: I5. Genotype: cdc13-1rad53-21 LEU+ in Y300 MATa + pOC56.

  • YS225 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1rad53-21 LEU+ in Y300 MATa. NO: I6.

  • YS226 ( Saccharomyces cerevisiae )

    NO: I7. Genotype: cdc13-1rad53-21 LEU+ in Y300 MATa + pOC58.

  • YS227 ( Saccharomyces cerevisiae )

    Genotype: Y300 + pRS416. NO: I8.

  • YS228 ( Saccharomyces cerevisiae )

    Genotype: Y300 + pOC56. NO: I9.

  • YS229 ( Saccharomyces cerevisiae )

    Genotype: Y300 + pOC57. NO: A1.

  • YS230 ( Saccharomyces cerevisiae )

    NO: A2. Genotype: Y300+ pOC58.

  • YS231 ( Saccharomyces cerevisiae )

    Genotype: Drad9::HIS3 in Y300 MATa (Y____)+ GAL-HACHK1 (pQL10). NO: A8.

  • YS232 ( Saccharomyces cerevisiae )

    NO: C5. Genotype: Diploid from cross rad53-21Dsic1:TRP1 in Y300 MATa(spore 1b from Deanna) x cdc13-1 in Y300 ALPHA LEU+.

  • YS233 ( Saccharomyces cerevisiae )

    NO: C7. Genotype: Drad9 + GAL:FlagRAD9 TRP (Stern) + GAL-HACHK1 (pQL10).

  • YS234 ( Saccharomyces cerevisiae )

    NO: C8. Genotype: cdc13-1Dchk1:HIS3Dcdh1:HIS3 in Y300 MATa haploid from cross: .

  • YS235 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1Dchk1:HIS3Dcdh1:HIS3 LEU+ MATalpha. NO: C9.

  • YS236 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1rad53-21Dsic1:TRP1 in Y300 MATa clone 8 haploid from YS232. NO: D1.

  • YS237 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1rad53-21Dsic1:TRP1 in Y300 Mata clone 2 LEU+ haploid from YS232. NO: D2.

  • YS238 ( Saccharomyces cerevisiae )

    Genotype: cdc13rad53-21Dcdh1:HIS3 MATa. NO: D3.

  • YS239 ( Saccharomyces cerevisiae )

    NO: D4. Genotype: rad53-21 + GAL-HACLB2:URA3 (pMT549 Tyers).

  • YS240 ( Saccharomyces cerevisiae )

    NO: D5. Genotype: Y300 + GAL-HACLB2:URA3 (pMT549 Tyers).

  • YS241 ( Saccharomyces cerevisiae )

    NO: D6. Genotype: DCHK1:HIS3 MATa (YS146) + GAL-HACLB2:URA3 (pMT549 Tyers).

  • YS242 ( Saccharomyces cerevisiae )

    Genotype: Ddun1:HIS3 MATa (MHY29) + GAL-HACLB2:URA3 (pMT549 Tyers). NO: D7.

  • YS243 ( Saccharomyces cerevisiae )

    NO: D8. Genotype: Dmec1::HIS3 GAP-RNR1:TRP in Y300 MATA (DES459) + GAL-HA:CHK1:URA3 (pQL10).

  • YS38 ( Saccharomyces cerevisiae )

    Genotype: rad53-31 loop-in into Y300 + pOSS446, using pYS18 (fragment containing rad53-31 mutation in pRS406) selected for URA+ . NO: C9.

  • YS39 ( Saccharomyces cerevisiae )

    Genotype: rad53-31 loop-in Y300, by transformation with pYS18 select URA+. NO: D1.

  • YS41 ( Saccharomyces cerevisiae )

    Genotype: loopout of YS39 5FOAr and HU sensitive. NO: D2.

  • YS5 ( Saccharomyces cerevisiae )

    NO: C3. Genotype: Dchk1:URA3 MATalpha Y300 ALPHA.

  • YS50 ( Saccharomyces cerevisiae )

    Genotype: loopout of YS38 5FOAr and HU sensitive. NO: D3.

  • YS51 ( Saccharomyces cerevisiae )

    NO: D4. Genotype: loopout of different isolate of YS38.

  • YS52 ( Saccharomyces cerevisiae )

    NO: D5. Genotype: loopout of different isolate of YS38.

  • YS53 ( Saccharomyces cerevisiae )

    NO: D6. Genotype: loopout of different isolate of YS38.

  • YS65 ( Saccharomyces cerevisiae )

    NO: D7. Genotype: mec1-1 (TWY308) + pJA98 GAL-HARAD53.

  • YS66 ( Saccharomyces cerevisiae )

    Genotype: mec1-1 (TWY308) + pSE556 (vector for pJA98). NO: D8.

  • YS67 ( Saccharomyces cerevisiae )

    NO: D9. Genotype: mec1-1 (TWY308) + pQL45 URA ADH-RAD53.

  • YS70 ( Saccharomyces cerevisiae )

    Genotype: cdc13-1Dchk1::HIS3 clone 1. Disruption of CHK1 in cdc13-1 LEU2+HIS3+ MATalpha in Y300 ALPHA. [cdc13-1 allele from TW… crossed into CRY by Hong and Brian]. NO: E1.

  • YS71 ( Saccharomyces cerevisiae )

    Genotype: same as YS70, different isolate clone 2. NO: E2.

  • YS79 ( Saccharomyces cerevisiae )

    Genotype: diploid cdc13-1Dchk1::HIS3 x YCH130 HIS3+TRP1+. NO: E3.

  • YS80 ( Saccharomyces cerevisiae )

    Genotype: diploid cdc13-1Dchk1::HIS3 x YCH125 HIS3+LYS+. NO: E4.

  • YS81 ( Saccharomyces cerevisiae )

    NO: E5. Genotype: different isolate same as YS80.

  • YS82 ( Saccharomyces cerevisiae )

    NO: E6. Genotype: different isolate same as YS80.

  • Δcds1 S. pombe ( Schizosaccharomyces pombe )

    Genotype: Frozen in YPD from Okayama. NO: C4.

  • Δcds1 S. pombe ( Schizosaccharomyces pombe )

    NO: C6. Genotype: Frozen in yeast extract (pombe media) from Okayama.

  • Δmec1 + CEN TEL1 ( Saccharomyces cerevisiae )

    NO: C7.

    Genotype: Dmec1 PDM197 haploid from YWJ60 (MEC+/Dmec1::HIS3) + pDM197 TEL1:URA:CEN endogenous promoter (Bill Jones).

Reagents

  • BAC vector ( BAC )

    BAC vector backbone for BACS containing hCHK1 locus.

  • BMH1 ( Plasmid )

    PCR ORF from Research Genetics into Topo vector (Invitrogen).

  • BMH2 ( Plasmid )

    PCR ORF from Research Genetics into Topo vector (Invitrogen).

  • C16 ( BAC )

    BAC clone from Genome systems containing hCHK1 locus by hybridization to pYS44 used for in situ and for genomic structure.

  • G9 ( BAC )

    Additional BAC vector from Genome systems containing hCHK1 locus.

  • GST-CDC25A ( Plasmid )

  • GST-CDC25B ( Plasmid )

  • GST-CDC25C ( Plasmid )

  • GST-CDC25C (200-256) ( Plasmid )

    From Helen Piwnica-Worms.

  • GST-CDC25C (200-256) S216A ( Plasmid )

    Mutagenesis of GST-CDC25C (200-256).

  • MIH1 ( Plasmid )

    PCR ORF from Research Genetics into Topo vector (Invitrogen).

  • PBlueBACHIS 2A ( BAC )

    Baculovirus vector from Invitrogen 4.9 Kb.

  • pBlueBACHIS 2B ( Plasmid )

    Same as 2A different reading frame.

  • pBlueBACHIS 2C ( Plasmid )

    Same as 2A, different reading frame.

  • pDKL2 ( Plasmid )

    HCHK1 cter fusion MycHis6 in pcDNA3.1 (-) 3 way XhoI-BHI pYS43 + BHI-EcoRI pYS58 (eliminate stop codon) + XhoI-EcoRI pcDNA3.1.

  • pDKL4 ( Plasmid )

    Chk1MycHis6 baculovirus transfer vector used to make His6 tagged Chk1 XbaI-PmnI from pDKL2-> XbaI-SmaI pVL1392.

  • pGEX in BL21 ( Plasmid )

    PGEX2Tcs in BL21. To be used as a GST only control.

  • pJS3 ( Plasmid )

    HA-CDC20 in YCplac33, CEN URA3.

  • pJS6 ( Plasmid )

    HA-CDC20 S52A S88A in YCplac33 CENURA3.

  • pMW034 ( Plasmid )

    HA-CDC20 in pRS415 (SmaI-SpeI fragment from pJS3).

  • pMW036 ( Plasmid )

    HA-CDC20 S52A S88A in pRS415 (SmaISpeI fragment from pJS6).

  • pQL10 ( Plasmid )

    GAL:HA-CHK1 pQL8 XhoI(B)-NotI into pSE556 R1(B)-NotI.

  • pQL11 ( Plasmid )

    pQL5 Nde-XhoI into pAS2 Nde-SalI.

  • pQL2 ( Plasmid )

    PCR scCHK1 from YES825 (primers 417/426) and cloned into TA (Spe-XhoI from this clone into BS Spe-XhoI = pQL4).

  • pQL5 ( Plasmid )

    YES825 Xho(kle)-Bam into pQL3 RV-Bam Replace 3’ end of scCHK1 gene with genomic fragment from YES825 scCHK1.

  • pQL6 ( Plasmid )

    YES7111 XhoI(B)-Bam into pQL4 RV-BamHI (BS). Replace 3’ end of gene with genomic fragment from YES7111 scCHK1.

  • pQL7 ( Plasmid )

    pQL5 into pGEX-2Tkcs by NdeI-XhoI GSTscCHK1.

  • pQL8 ( Plasmid )

    (3 way) pETX-HA Nde-PstI + pETX-HA NotI-PstI + pQL5Nde-NotI.

  • pQL9 ( Plasmid )

    GAL:scCHK1 pQL5 into pSE556 by R1-NotI.

  • pYS1 ( Plasmid )

    GAL:rad53-21 BamHI-Not1 from pAA1-> BamHI-Not1 pJA98.

  • pYS10 ( Plasmid )

    rad53-31 in pBSKII(-), mutagenesis of pYS8 using primer 502 K227A ATP binding domain.

  • pYS17 ( Plasmid )

    GAL: HArad53-31 BglII-NotI from pYS10->BglII-NotI incomplete digest of pJA98.

  • pYS18 ( Plasmid )

    Transplacement vector for rad53-31 BsteII-NotI from pYS10 -> pRS406.

  • pYS2 ( Plasmid )

    GAL:rad53-21 BH1-Not1 from pWJ21-> BH1-Not1 pJA98.

  • pYS21 ( Plasmid )

    Rad53-31 under its own promoter in pRS415 (3 way) SacI-BglII from pJA93 + BglII-NotI pYS10-> SacI-NotI pRS415.

  • pYS22 ( Plasmid )

    rad53-31 under endogenous pormoter in pRS425.

  • pYS23 ( Plasmid )

    rad53-31 under endogenous promoter in pRS426.

  • pYS24 ( Plasmid )

    GSTHARad53 in pGEX2Tcs NcoI-BHI from pQL41 ->NcoI BHI pGEX2Tcs (3way).

  • pYS26 ( Plasmid )

    HARad53-> pADHGST NcoI-BHI from pQL41 -> NcoI-BHI pADH GST (3 way).

  • pYS27 ( Plasmid )

    RAD53 under endogenous promoter in pRS415 SacI-BglII pJA93 + BglII-NotI pWJ26 -> SacI-NotI pRS415.

  • pYS3 ( Plasmid )

    scCHK1 XhoI genomic fragment from lambda YES clone -> XhoI pRS415.

  • pYS35 ( Plasmid )

    XhoI Genomic fragment from lambda YES clone 8-2-5 containing CHK1 -> XhoI pRS414 insert ~4.2 Kb insert.

  • pYS36 ( Plasmid )

    Same as pYS35 but opposite orientation.

  • pYS37 ( Plasmid )

    ScCHK1 in Litmus 29 XbaI-XhoI from pQL5 -> Xba-XhoI Litmus 29.

  • pYS39 ( Plasmid )

    GAL:scCHK1 in pMW29 SacI-BglII from pYS37->SacI-BHI pMW29, URA3 ADE3.

  • pYS4 ( Plasmid )

    rad53 site directed mutant T354A site directed mutagenesis using primer 505 on pYS8.

  • pYS41 ( Plasmid )

    Harad53-31 mutagenesis of PQL41 K227A.

  • pYS42 ( Plasmid )

    Incomplete hCHK1 cDNA from pACT library clone 5-3-1 XhoI fragment->pBSKSII.

  • pYS43 ( Plasmid )

    Complete hCHK1 cDNA NotI-BglII from pYS42 ->BlgII-NotI of pYS44.

  • pYS44 ( Plasmid )

    Incomplete hCHK1 cDNA from pACT library clone 9-2-2 XhoI fragment -> pBSKSII.

  • pYS45 ( Plasmid )

    GST-hCHK1 in pGEX2tcs NdeI-BglII pYS47 (PCR mutagenesis to introduce NdeI) + BglII-EcoRI from pYS44-> Nde-EcoRI pGEX2Tcs.

  • pYS51 ( Plasmid )

    SmaI fragment from pJA5_ containing HISKAN -> EcoRV pYS49 (Xho fragment containing CHK1->SalI cut pUC19). Transplacement construct for chk1 disruption.

  • pYS62 ( Plasmid )

    GST-hCHK1 in pGEX2Tcs alternative for pYS45 send this clone out.

  • PYS63 ( BAC )

    GST-hCHK1 in pvL1393 baculovirus transfer vector, used to make GSTChk1 expressing virus.

  • pYS64 ( Plasmid )

    D330A kinase mutant hChk1 in pBSKSII 3 way XhoI-XmnI pYS57 (mutagenized fgmt) +XmnI-KpnI pYS44 +XhoI-XmnI pBSKSII (-).

  • pYS65 ( Plasmid )

    D330A kin- hCHK1 in pGEX2Tcs NdeI-EcoRI pYS62-> NdeI-EcoRI pGEX2Tcs.

  • pYS67 ( Plasmid )

    Mouse CHK1 cDNA in pACT clone # 7241 isolated by screening murine lambda ACT library with hCHK1 cDNA.

  • pYS68 ( Plasmid )

    MCHK1 cDNA in pBluescript XhoI fragment from pYS67-> XhoI pBluescript NotI-CHK1-KpnI.

  • pYS69 ( Plasmid )

    Same as pYS68 but opposite orientation KpnI-CHK1-NotI.

  • pYS70 ( Plasmid )

    PYS68delta KpnI fragment used for in situ hybridization of mouse embryos.

  • pYS71 ( Plasmid )

  • pYS72 ( Plasmid )

    CMV:hCHK1 cDNA in pTracer from Invitrogen.

  • pYS73 ( Plasmid )

    CMV:hCHK1 D330A in pTracer.

  • pYS74 ( Plasmid )

    CMV:mCHK1 in pTracer.

  • pYS77 ( Plasmid )

    GAL:scCHK1 kin- mutagenesis of pQL10.

  • pYS8 ( Plasmid )

    Rad53 in pBSKII(-) KpnI-XbaI fragment from pWJ26 -> pBSKII(-) KpnI-XbaI.

Research Opportunities

  • Student research opportunities ( Research Opportunity )

    The Sanchez Lab participates in undergraduate and graduate student research opportunity programs, e.g. Howard Hughes Medical Institute (HHMI), Women in Science Project at Dartmouth, Dartmouth Presidential Scholar Program.


Web Links:

Last updated: 2012-05-08T10:51:38.877-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016