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Kisselev Laboratory

Summary:

The Kisselev laboratory works on fundamental and translational aspects of proteasome pharmacology, biochemistry, and cell biology. Proteins in all living cells are constitutively synthesized and degraded. The 26S proteasome is a large (2.5MDa) ATP-dependent proteolytic machine, which is responsible for the majority of protein degradation in mammalian cells. The majority of its substrates are damaged and misfolded proteins. Cancer cells, especially those which produce large amounts of proteins (e.g., antibody-secreting multiple myeloma cells), generate excessive amounts of abnormal proteins resulting in a high load on proteasome in these cells and making cancer cells highly sensitive to proteasome inhibitors. Peptide boronate inhibitor of proteasome Velcade (bortezomib) is being used for the treatment of multiple myeloma and mantle cell lymphoma, and five other proteasome inhibitors are now undergoing clinical trials.

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Reagents

  • 5T33MM ( Tumor-derived cell line )

  • A549 ( Tumor-derived cell line )

  • BT-20 ( Tumor-derived cell line )

    Notes: triple-negative

  • BT-474 ( Tumor-derived cell line )

    Notes: ER+, HER2+

  • BT-549 ( Tumor-derived cell line )

    Notes: triple-negative

  • ED-1 ( Tumor-derived cell line )

    Notes: Mouse; generated in Dmitrovsky lab from Cyclin E transgenic lung tumor

  • EL-4 ( Tumor-derived cell line )

    Notes: Mouse

  • H226 ( Tumor-derived cell line )

  • H23 ( Tumor-derived cell line )

  • HBL-100 ( Tumor-derived cell line )

  • HCC-1187 ( Tumor-derived cell line )

    Notes: triple-negative

  • HCC-1937 ( Tumor-derived cell line )

    Notes: triple-negative

  • HCC-38 ( Tumor-derived cell line )

    Notes: triple-negative

  • HeLA ( Tumor-derived cell line )

  • HeLa S3 ( Tumor-derived cell line )

  • Hop62 ( Tumor-derived cell line )

  • KMS-11 ( Tumor-derived cell line )

  • KMS-12-BM ( Tumor-derived cell line )

  • KMS-18 ( Tumor-derived cell line )

  • KMS-21-PE ( Tumor-derived cell line )

  • MCF-10A ( Cell line )

    Notes: immortalized epithelial

  • MCF-7 ( Tumor-derived cell line )

    Notes: ER+

  • MDA-MB-231 ( Tumor-derived cell line )

    Notes: triple-negative

    Tissue/Cancer Type: Melanoma?

  • MDA-MB-435 ( Tumor-derived cell line )

    Notes: originally thought to be breast, but now melanoma? People are avoiding working with it.

  • MDA-MB-453 ( Tumor-derived cell line )

    Notes: triple-negative

  • MDA-MB-468 ( Tumor-derived cell line )

    Notes: triple-negative

  • MM1.R ( Tumor-derived cell line )

  • MM1.S ( Tumor-derived cell line )

  • NCI-H929 ( Tumor-derived cell line )

  • OPM-2 ( Tumor-derived cell line )

  • RPMI-8226 ( Tumor-derived cell line )

  • RPMI-8226/Dox6 ( Tumor-derived cell line )

  • RPMI-8226/LR5 ( Tumor-derived cell line )

  • SK-BR-3 ( Tumor-derived cell line )

    Notes: HER2+; not sure whether frozen stock is OK

  • SUM-102 ( Tumor-derived cell line )

    Notes: not sure whether frozen stock is OK

  • SUM-149 ( Tumor-derived cell line )

  • T47D ( Tumor-derived cell line )

    Notes: ER+

  • U266 ( Tumor-derived cell line )

  • ZR-75-1 ( Tumor-derived cell line )

    Notes: ER+


Last updated: 2013-05-01T08:57:37.661-04:00

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