eagle-i Dartmouth CollegeDartmouth College
See it in Search

Shared Equipment Rubin 6 and 7

Summary:

Dartmouth researchers have access to 37 Shared Resource instruments which are housed in various labs throughout the 6th and 7th floors of the Rubin building.

Please note:
See individual instruments and protocols for location and the lab responsible.
No cost is involved; be sure to clean up when finished.
The "responsible lab" provides necessary instrument training.

Affiliations:

People:

    Resources:

    Instruments

    • Biacore X100 (Surface Plasmon Resonance) ( Surface plasmon resonance instrument )

      Location: Rubin 6th floor, Bench 76
      Responsible lab: Spaller

    • Cell counter ( Instrument )

      Location: Rubin 6th floor, in Shared Resource Equipment Rm 649

      Lab responsible: Shared Equipment

    • Cell counter ( Automatic cytometer )

    • Centrifuge ( Centrifuge )

      Location: Rubin 764O3 near Bench 21

    • Centrifuge-Tabletop ( Centrifuge )

      Location: Rubin 6th floor, In hall near Cold Rm 664O5
      Lab responsible: Kisselev

    • Centrifuge-Tabletop ( Instrument )

      Located: Rubin 6th floor, near Bench 61
      Lab responsible: Danilov

    • Chemidoc ( Chemiluminescence analyzer )

      Located: Rubin 7th floor, Bench 1
      Responsible lab: Shared Equipment

    • Cryostat ( Cryofixation device )

      Located: Rubin 7th floor, Bench 16
      Responsible lab: Israel

    • Cytospin machine ( Instrument )

      Located: Rubin 6th floor, Bench 105
      Lab responsible: Brinckerhoff

    • Darkroom Film Processor ( X-ray film processor )

      Location: Room 764N6 on main 7th floor hall of Rubin
      Responsible lab: Shared Equipment

    • Floor centrifuge ( Centrifuge )

      Location: Rubin 7 near Cold rm 664O5

    • Fluorescent microscope ( Microscope )

      Located: Rubin 6th floor, in Procedure Rm 663L2, near Autoclave Rm
      Responsible Lab: Brinckerhoff

      Olympus 1X50

    • Fluorescent microscope ( Fluorescence microscope )

      Located: 765P4 in office area on 7th floor Rubin
      Responsible lab: Israel

    • Geldoc ( Chemiluminescence analyzer )

      Located: on Bench 2, 7th floor Rubin Responsible lab: Israel lab

    • Incubator ( Incubator )

      Located: Rubin 6th floor, in Equipment Alcove 664L4 near Bench 61
      Responsible lab: Danilov

    • Incubator oven ( Incubator )

      Location: Hallway 7

    • Incubator shaker ( Incubator shaker )

      Location: Hallway 7

    • Infrared Imager ( Imager )

      Located: Rubin 6th floor, Bench 88
      Responsible lab: Kisselev

    • Li-Cor C Digit Blot Scanner ( Instrument )

      Located: Rubin 6th floor, Genomics Shared Instrument Rm649 in main hall
      Lab responsible: Shared Equipment

    • LMaxII Plate Reader ( Instrument )

      Located: Rubin 6th floor, Bench 112
      Responsible lab: Kisselev

    • Microplate Reader ( Instrument )

      Located: Rubin 6th floor, in Equip. alcove 664L4 near Bench 61
      Responsible lab: Danilov

    • Minus 20C freezer ( Freezer )

      Located: Rubin 7th floor hallway, across from bathrooms.

    • Minus 80C freezer ( Ultra low temperature freezer )

      Located: Rubin 6th floor hallway
      Responsible lab: Shared Equipment

    • My IQ cycler ( Real-time PCR machine )

      Located: Rubin 7th floor, Bench 2
      Responsible lab: Israel Lab

      Note: this unit is on Emergency Power and the computer has a UPS to prevent loss of data during power outages.

    • Nanodrop ( Spectrophotometer )

      Located: Rubin 6th floor Genomics Shared Instrument Room 649
      Responsible Lab: Shared equipment

      Note: Please bring your own Kim wipes for the Nanodrop.

    • Nanodrop ( Spectrophotometer )

      Located: Rubin 7th floor Bench 2
      Responsible Lab: Shared equipment

      Note: Please bring your own Kim wipes for the Nanodrop.

    • Optima ultracentrifuge ( Ultracentrifuge )

      Located: Rubin 6th floor Bench 107
      Responsible Lab: Kisselev

    • Photospectrometer ( Spectrophotometer )

      Located: Rubin 6th floor in Procedure Rm 663L2 near Autoclave rm
      Responsible Lab: Brinkerhoff

    • Probe Sonicator ( Sonicator )

      Located: Rubin 6th floor, in Fume Hood Alcove 663N5 near Bench 105
      Responsible Lab: Lowrey

    • qPCR ( Real-time PCR machine )

      Located: Bench 2, 7th floor

    • qPCR ( Real-time PCR machine )

      Located: Genomics Sh Instrument Rm

    • Qubit ( Fluorometer )

      Location: Rubin 6th floor, Genomics Shared Instrument Rm 649
      Responsible Lab: Tomlinson

    • RNA/DNA analyzer ( Spectrophotometer )

      Located: Bench 2 Rubin 7th floor
      Responsible Lab: Shared equipment

      Note: Please bring your own Kim wipes for the Nanodrop.

    • Robo-cycler ( Thermal cycler )

      Located: Rubin 7th floor, Bench 1
      Responsible lab: Shared Equipment

    • Scintillation counter ( Scintillation counter )

      Wallac MicroBeta® TriLux

      Location: Rubin 6th floor, Bench 107
      Responsible Lab: Kisselev

    • Sonicator ( Sonicator )

      Sonics Vibra Cell

      Location: Rubin 6th floor, in Cold Rm 663N4
      Responsible Lab: Cole

    • Sorvall floor centrifuge ( Centrifuge )

      Location: Rubin 6th floor, near Cold Rm 664O5
      Responsible lab: Shared Instrument

    • Sorvall floor centrifuge ( Centrifuge )

      Located: Rubin 7th floor near Cold Rm 764O5
      Responsible lab: Shared Equipment

    • Sorvall RC5CH Centrifuge ( Centrifuge )

      Located: 764O3 near Bench 21
      Responsible lab: Israel
      Rubin 7

    • SpectraMax Plate reader ( Spectrophotometer )

      Location: Rubin 6th floor TC Rm near Fishbowl
      Responsible lab: Kisselev

    • Speed Vac ( Vacuum centrifuge concentrator )

      Location: Shared
      Bench 1(7)

    • TA Nano ITC (Isothermal Titration Calorimeter) ( Isothermal titration calorimeter )

      Location: Rubin 6th floor, Bench 79
      Responsible lab: Spaller

    • Tabletop centrifuge ( Tabletop centrifuge )

      Located: Rubin 7th floor, Bench 2
      Responsible lab: Shared Equipment

    • Thermal cycler C1000 ( Thermal cycler )

      Location: Rubin 6th floor Genomics Sh Instrument Rm 649
      Responsible lab: Shared equipment

    Protocols

    • -20C Freezer Use Protocol.docx ( Protocol )

      1. Email Kathy Smith to determine if the NCCC -20C freezer is available. Kathleen.M.Smith@dartmouth.edu

      2. Prepare to use two to three hours to empty your entire freezer.

      3. Ensure that all sample boxes have adequate identification: researcher’s initials, date, lab name.

      4. Wearing appropriate PPE (cryogenic gloves, eyewear), bring a maximum of three to four racks of your samples to the freezer on a cart and load them into the NCCC -20C freezer.

      5. Avoid overloading the freezer with warm specimens. The NCCC -20C unit is not a “rapid-freeze” device. Freezing large quantities of liquid, or high-water content items, will temporarily increase the cabinet temperature and cause the compressor to operate for a prolonged time period. Even though the freezer may only rise to -10C, the freezer continues to warm after you close the door, oftentimes rising to -0C. Allow 2 hours for temperature to continue to rise and then drop back down to -20C prior to adding any additional racks.

      6. Avoid opening the freezer door for prolonged time periods. Cabinet temperature air escapes rapidly and is replaced with higher humidity room temperature air, which results in greater frost build-up on the doors and gasket.

      7. Do not place loose test tubes or cryovial racks with specimens in the freezers.

      8. Be sure to remove all of your samples from the -20C freezer when your freezer is repaired. Check in the snowy corners.


      9. Email Kathy Smith when you are finished using the NCCC -20C freezer. She will then notify the next lab that the freezer is available. Kathleen.M.Smith@dartmouth.edu

    • -80 Freezer Use Protocol.docx ( Protocol )

      1. Email Kathy Smith to determine if the NCCC -80C freezer is available Kathleen.M.Smith@dartmouth.edu.

      2. Prepare to use five to six hours to empty your entire freezer.

      3. Ensure that all sample boxes have adequate identification: researcher’s initials, date, lab name.

      4. Wearing appropriate PPE (cryogenic gloves, eyewear), bring a maximum of three to four racks of your samples to the freezer on a cart and load them into the NCCC -80C freezer.

      5. Avoid overloading the freezer with warm specimens. The NCCC -80C unit is not a “rapid-freeze” device. Freezing large quantities of liquid, or high-water content items, will temporarily increase the cabinet temperature and cause the compressor to operate for a prolonged time period. Even though the freezer may only rise to -60C, the freezer continues to warm after you close the door, oftentimes rising to -45C. Allow 3 hours for temperature to continue to rise and then drop back down to -80C prior to adding any additional racks.

      6. If you are defrosting your -20, add only one to two racks of -20C specimens to the freezers, allowing ample time between batches for the freezer cabinet temperature to recover to operating temperature.

      7. Avoid opening the freezer door for prolonged time periods. Cabinet temperature air escapes rapidly and is replaced with higher humidity room temperature air, which results in greater frost build-up on the doors and gasket.

      8. Do not place loose test tubes or cryovial racks with specimens in the freezers.

      9. Once your freezer is ready, be sure to remove all of your samples from the -80C freezer. Check in the snowy corners.

      10. Ensure that your freezer filter is cleaned 3-4 times a year which will lengthen the life of your freezer, thereby reducing the number of times you will need the NCCC -80C freezer.

      11. Email Kathy Smith when you are finished using the NCCC -80C freezer. She will then notify the next lab that the NCCC -80 freezer is available.


    Last updated: 2015-06-11T17:01:27.276-04:00

    Copyright © 2016 by the President and Fellows of Harvard College
    The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016